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565758

β-Secretase Substrate IV, Fluorogenic

≥95% (HPLC), lyophilized solid, β-secretase substrate, Calbiochem®

Synonym(s):

β-Secretase Substrate IV, Fluorogenic, FS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic

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Size/SKUAvailabilityPrice
1 mg

Available to ship TODAYfromAreál Kühne+Nagel spol. s r.o.

5 900,00 CZK

About This Item

Empirical Formula (Hill Notation):
C91H129N25O25S
CAS Number:
310427-94-2
Molecular Weight:
2005.22
MDL number:
MFCD03093396
NACRES:
NA.77
UNSPSC Code:
12352202
Assay:
≥95% (HPLC)
Form:
lyophilized solid
Storage condition:
OK to freeze, protect from light

5 900,00 CZK

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Product Name

β-Secretase Substrate IV, Fluorogenic, A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP).

Quality Segment

assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

color

red

solubility

DMSO: 5 mg/mL

fluorescence

λex ~350 nm, λem ~490 nm

shipped in

ambient

storage temp.

−20°C

General description

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm; emission max.: ~490), based on the GVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by β-secretase results in fluorescence enhancement. Reported Kcat/km values (min-1/µM) for pro-mempasin 2 and its proteolytically activated species Leu28p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37°C).

Biochem/physiol Actions

Product does not compete with ATP.
Reversible: no
Cell permeable: no
Primary Target
β-secretase substrate

Packaging

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.

Other Notes

Ermolieff, J., et al. 2000. Biochemistry 39, 12450.
H-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item
565767565764444256
β-Secretase Substrate IV, Fluorogenic A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP).

Sigma-Aldrich

565758

β-Secretase Substrate IV, Fluorogenic

α-Secretase Substrate II, Fluorogenic An internally quenched fluorogenic peptide substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP).

Sigma-Aldrich

565767

α-Secretase Substrate II, Fluorogenic

γ-Secretase Substrate, Fluorogenic An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase.

Sigma-Aldrich

565764

γ-Secretase Substrate, Fluorogenic

MMP Substrate III, Fluorogenic A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination.

Sigma-Aldrich

444256

MMP Substrate III, Fluorogenic

description

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP).

description

An internally quenched fluorogenic peptide substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP).

description

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase.

description

A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination.

form

lyophilized solid

form

solid

form

lyophilized

form

lyophilized solid

assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥97% (HPLC)

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

DMSO: 5 mg/mL

solubility

DMSO: 5 mg/mL

solubility

DMSO: 5 mg/mL, 5% acetic acid: soluble

solubility

Tris-buffered saline, pH 7.6: soluble, DMSO: soluble

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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