407707

PhosphoDetect Anti-Insulin Receptor (pTyr^1162/1163) Rabbit pAb

liquid, Calbiochem^®

• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: polyclonal
• Species reactivity: human
• Application: —
• Citations: 9

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• isotype: IgG
• shipped in: wet ice
• storage temp.: −70°C
• target post-translational modification: phosphorylation (pTyr1162/pTyr1163)
• Gene Information: human ... INSR(3643)

Description

General description

Note: 1 T = 1 test. Sufficient for 10 Western mini blots.

Rabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the insulin receptor (IR) protein phosphorylated at Tyr^1162/1163 and insulin-like growth factor 1 receptor (IGF-1R) protein phosphorylated at Tyr^1162/1163.

Recognizes the ~95 kDa β subunit of the insulin receptor protein phosphorylated at Tyr¹¹⁶² and Tyr¹¹⁶³ in insulin receptor transfected cells. Cross-reacts with the insulin-like growth factor-1 receptor phosphorylated at Tyr¹¹³⁵ and Tyr¹¹³⁶.

This PhosphoDetect Anti-Insulin Receptor (pTyr^1162/1163) Rabbit pAb is validated for use in ELISA, WB, ICC for the detection of Insulin Receptor (pTyr^1162/1163).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr^1162/1163 phosphorylation sites of human insulin receptor

Application

ELISA (see comments)

Immunoblotting (1:1000)

Immunocytochemistry (see comments)

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Bevan, P. 2001. J. Cell Sci.114, 1429.
Ottensmeyer, F.P., et al. 2000. Biochemistry39, 12103.
Virkamaki, A., et al. 1999. J. Clin. Invest.103, 931.
Schmid, E., at al. 1998. FASEB J.12, 863.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem.262, 15341.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA83, 328.
White, M.F. and Kahn, C.R. 1986. The Enzymes17, 247.
Ebina, Y., et al. 1985. Cell40, 747.
Ganguly, S., et al. 1985. Current Topics in Cellular Regulation27, 83.
Grunberger, G., et al. 1984. Science223, 932.

The immunogen sequence is 100% conserved in the mouse and rat insulin recptor protein, but cross-reactivity has not been tested. This antibody has also been reported to work for ELISA and immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable