407306

γ-Interferon, Human, Recombinant, E. coli

• NACRES: NA.77
• UNSPSC Code: 12352202
• Form: liquid

Properties

• description: Merck USA index - 14, 4992
• Quality Segment: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Recombinant, human γ-interferon expressed in E. coli. A multi-functional protein consisting of 144 amino acids. Exhibits antiviral and antitumor properties. Regulates the development of specific immune responses. Expressed in and purified from E. coli.

Biochem/physiol Actions

ED₅₀ = 0.5-3.0 ng/ml as measured by inhibition (cytostasis) of human WiDr cells.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

Sterile-filtered (0.22 µm) in 40 mM Tris-HCl, pH 7.4, carrier-free.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C). Do not store in glass vials without addition of carrier protein. Further dilute in low endotoxin medium or buffer containing FBS or tissue culture grade BSA.

Other Notes

Kohji, K., et al. 1994. J. Int. J. Cancer 58, 380.
Kolb, J.P., et al. 1994. J. Biol. Chem.269, 9811.
Müller, U., et al. 1994. Science264, 1918.
Zahedi, K., et al. 1994. J. Biol. Chem.269, 9669.
Shuai, K., et al. 1993. Science261, 1744.
Wilson, K.C., and Finbloom, D.S. 1992. Proc. Natl. Acad. Sci. USA89, 11964.
Meager, A. 1987. Lymphokines and Interferons, A Practical Approach (Clemens, M.J., et al., eds.) p. 129, IRL Press, Oxford.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable