345804

β1-3,6-Galactosidase, Recombinant, E. coli

• Synonym(s): β1-3,6-Galactosidase, Recombinant, E. coli, β1-3,6-D-Galactoside Galactohydrolase
• CAS Number: 9031-11-2
• EC Number: 3.2.1.23 (BRENDA, IUBMB)
• NACRES: NA.54
• UNSPSC Code: 12352202
• MDL number: MFCD00009634
• Specific activity: ≥30 units/mL, ≥75 units/mg protein
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• Quality Segment: 100
• form: liquid
• specific activity: ≥30 units/mL, ≥75 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• foreign activity: Proteases, none detected
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Recombinant, β1-3,6-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,3- and β1,6-linked galactose from complex carbohydrates and glycoproteins. Not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Other Notes

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.

Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 12 - Non Combustible Liquids