218800

Caspase-7, Human, Recombinant, E. coli

Caspase-7, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

• Synonym(s): Mch3, ICE-LAP3
• UNSPSC Code: 12352202
• Specific activity: ≥15,000 units/mg protein
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• Quality Segment: 100
• form: lyophilized
• specific activity: ≥15,000 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

M.W. 12,000 and 20,000 (heterodimer).

Recombinant, human caspase-7 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 12000 and 20000.

Preparation Note

Following reconstitution, aliquot and freeze (-70°C). Stock solitions are stable for up to 3 months at -70°C.

Reconstitute to 1 unit/µl in PBS, 15% glycerol.

Other Notes

One unit is defined as the amount of enzyme that will cleave 1 nmol of Ac-DEVD-pNA (Cat. No. 235400) per hour at 37°C, pH 7.2.

Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem.272, 25719.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable