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Anti-CD18 Mouse mAb (IB4)

Name: Anti-CD18 Mouse mAb (IB4)
Brand: MM
Price: 10700 CZK
Availability: InStock

liquid, ≥95% (SDS-PAGE), clone IB4

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Synonym(s):

Anti-β2 Integrin

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Size/SKU	Availability	Price
100 μg	Available to ship TODAYfromAreál Kühne+Nagel spol. s r.o.	10 700,00 CZK

About This Item

NACRES:

NA.43

UNSPSC Code:

12352203

Clone:

IB4, monoclonal

Species reactivity:

human

Application:

—

Citations:

10 700,00 CZK

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Properties

biological source

mouse

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

IB4, monoclonal

assay

≥95% (SDS-PAGE)

form

liquid

contains

0.5 mg/mL gentamicin sulfate as preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

dilution

(Flow Cytometry (5-10 µg/mL)
Frozen Sections
Immunoprecipitation
Cell Inhibition Assays )

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Anti-CD18, mouse monoclonal, clone IB4, recognizes the ~95 kDa β2 integrin subunit. It is validated for FC, IP, IHC on frozen sections, and cell inhibition assays.

Protein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18).

Recognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.

Immunogen

Human

human peripheral blood monocytes

Application

Flow Cytometry (5-10 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

Cell Inhibition Assays (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Preparation Note

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Analysis Note

Negative Control
Nalm-6 cells

Positive Control
HPB-MLT cells

Other Notes

Berman, M.E., et al. 1996. J. Immunol.156, 1515.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995. Haematologica80, 161.
Kaneko, M., et al. 1995. J. Immunol.155, 2631.
Landis, R.C., et al. 1994. J. Cell Biol.126, 529.
Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5699.

Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x10⁵ cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item	OP16	CP53	OP107
Sigma-Aldrich 217660 Anti-CD18 Mouse mAb (IB4)	Sigma-Aldrich OP16 Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4)	Sigma-Aldrich CP53 Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1)	Sigma-Aldrich OP107 Anti-BRCA1 (Ab-4) Mouse mAb (SD118)
description liquid, ≥95% (SDS-PAGE), clone IB4	description liquid, clone 7.16.4, Calbiochem^®	description liquid, clone W-CAM-1, Calbiochem^®	description liquid, clone SD118, Calbiochem^®
antibody form purified antibody	antibody form purified antibody	antibody form purified antibody	antibody form purified antibody
species reactivity human	species reactivity rat, human	species reactivity human	species reactivity human
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone IB4, monoclonal	clone 7.16.4, monoclonal	clone W-CAM-1, monoclonal	clone SD118, monoclonal
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 100	Quality Level 200	Quality Level 100	Quality Level 100

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Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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