208783

Calmodulin, Porcine Brain

Calmodulin, Porcine Brain, is a ubiquitous Ca²⁺-binding protein that serves as a physiological effector of a wide range of biological processes. Purified from porcine brain.

• NACRES: NA.77
• UNSPSC Code: 12352202
• Form: lyophilized
• Assay: >98% (SDS-PAGE)

Properties

• Quality Segment: 100
• assay: >98% (SDS-PAGE)
• form: lyophilized
• specific activity: ≥35,000 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• solubility: water: 1 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Calmodulin (CaM) purified from porcine brain. A ubiquitous, calcium-binding protein that interacts with and regulates a wide variety of proteins, thus affecting different cellular functions and calcium-calmodulin-dependent signaling pathways. CaM mediates processes like inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth, and the immune response. Useful for applications requiring active pure calmodulin.

Physical form

Lyophilized from 10 mM sodium phosphate buffer, 5% sucrose, pH 7.0.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Massom, L., et al. 1990. Biochemistry29, 671.

One unit is defined as the amount of calmodulin that will stimulate 0.01 unit of 3′5′-cyclic nucleotide phosphodiesterase to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 µM Ca²⁺ in a 3 ml reaction volume at 30°C, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1