208780

Calmodulin, Biotinylated, Porcine Brain

• UNSPSC Code: 12352202

Properties

• assay: >98% (SDS-PAGE)
• Quality Segment: 100
• form: lyophilized
• specific activity: ≥35,000 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• solubility: water: 1 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Calmodulin (CaM) purified from porcine brain and biotinylated using NHS ester-activated biotin with an 8 atom spacer arm. A ubiquitous, calcium-binding protein that interacts with and regulates a wide variety of proteins, thus affecting different cellular functions and calcium-calmodulin-dependent signaling pathways. CaM mediates several biological processes, such as inflammation, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth, and the immune response. Useful for enzyme activation, CaM binding studies, and general assessment of CaM-dependent signaling pathways. Biotin/Protein Ratio: 0.5-1.0 as determined by HABA assay.

Physical form

Lyophilized from 10 mM sodium phosphate buffer, 5% sucrose, pH 7.0.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Billingsley, M.L., et al. 1985. Proc. Natl. Acad. Sci. USA82, 7585.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10-13 - German Storage Class 10 to 13