208750

ALLN

InSolution, ≥95%, Inhibitor of calpain I, calpain II, cathepsin B, and cathepsin L

• Synonym(s): InSolution ALLN, Calpain Inhibitor I, LLNL, MG 101, Proteasome Inhibitor V
• Empirical Formula (Hill Notation): C₂₀H₃₇N₃O₄
• CAS Number: 110044-82-1
• Molecular Weight: 383.53
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD00065505
• Assay: ≥95% (HPLC)
• Form: solution
• Storage condition: OK to freeze, protect from light

Properties

• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: solution
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: clear colorless
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: N([C@@H](CC(C)C)C(=O)N[C@@H](CCCC)C=O)C(=O)[C@@H](NC(=O)C)CC(C)C
• InChI: 1S/C20H37N3O4/c1-7-8-9-16(12-24)22-19(26)18(11-14(4)5)23-20(27)17(10-13(2)3)21-15(6)25/h12-14,16-18H,7-11H2,1-6H3,(H,21,25)(H,22,26)(H,23,27)/t16-,17-,18-/m0/s1
• InChI key: FMYKJLXRRQTBOR-BZSNNMDCSA-N

Description

General description

A 10 mM solution of ALLN (Cat. No. 208719) in anhydrous DMSO. Inhibitor of calpain I (K_i = 190 nM), calpain II (K_i = 220 nM), cathepsin B (K_i = 150 nM), and cathepsin L (K_i = 0.5 nM). Inhibits neutral cysteine proteases and proteasome (K_i = 6 µM). Protects against neuronal damage caused by hypoxia and ischemia. Inhibits apoptosis in thymocytes and metamyelocytes. Inhibits cell cycle progression at the G₁/S border and metaphase/anaphase in CHO cells by inhibiting cyclin B degradation. Also prevents nitric oxide production by activated macrophages by interfering with transcription of the inducible nitric oxide synthase gene. Inhibits the proteolysis of IkB-α and IkB-β by the ubiquitin-proteasome complex.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
calpain 1

Product does not compete with ATP.

Reversible: no

Target K_i: 190 nM, 220 nM, 150 nM, 500 pM, against calpain I, calpain II, cathepsin B, and cathepsin L, respectively

Packaging

Packaged under inert gas

Physical form

A 10 mM (5 mg/1.30 ml) solution of ALLN (Cat. No. 208719) in DMSO.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Milligan, S.A., et al. 1996. Arch. Biochem. Biophys.335, 388.
Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun.215, 721.
Squier, M.K., et al. 1994. J. Cell Physiol.159, 229.
Rami, J., and Kreiglstein, J. 1993. Brain Res.609, 67.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Flammable (J)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: 188.6 °F - closed cup
• flash_point_c: 87 °C - closed cup