Stock solutions of this product can be made in DMSO to a concentration of 100 µM. Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 year at -20°C. Just before use, the stock solution should be serially diluted in DMSO to a working stock that is at a 1000-fold higher concentration than the concentration desired in the final aqueous reaction mix or culture medium.
196000
Bafilomycin A1
from Streptomyces griseus, ≥90% (HPLC), film, V-ATPase inhibitor
Synonym(s):
Bafilomycin A1, Streptomyces griseus
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| Size/SKU | Availability | Price |
|---|---|---|
10 μg | Please contact Customer Service for Availability | CZK 4,990.00 |
1 set | Please contact Customer Service for Availability | CZK 11,900.00 |
About This Item
Empirical Formula (Hill Notation):
C35H58O9
CAS Number:
Molecular Weight:
622.83
UNSPSC Code:
12352200
NACRES:
NA.77
MDL number:
Assay:
≥90% (HPLC)
Form:
film
Storage condition:
OK to freeze, protect from light
CZK 4,990.00
Please contact Customer Service for Availability
Product Name
Bafilomycin A1, Streptomyces griseus,
assay
≥90% (HPLC)
Quality Segment
form
film
shelf life
3 yr
storage condition
OK to freeze, protect from light
solubility
DMSO: 100 μM, ethanol: soluble
storage temp.
−20°C
SMILES string
O1[C@@H]([C@H]([C@@H](C[C@]1(O)[C@H]([C@H](O)[C@@H]([C@H]2OC(=O)\C(=C\C(=C\[C@H]([C@H]([C@H](C\C(=C\C=C\[C@@H]2OC)\C)C)O)C)\C)\OC)C)C)O)C)C(C)C
InChI
1S/C35H58O9/c1-19(2)32-24(7)27(36)18-35(40,44-32)26(9)31(38)25(8)33-28(41-10)14-12-13-20(3)15-22(5)30(37)23(6)16-21(4)17-29(42-11)34(39)43-33/h12-14,16-17,19,22-28,30-33,36-38,40H,15,18H2,1-11H3/b14-12+,20-13+,21-16+,29-17-/t22-,23+,24-,25-,26-,27+,28-,30-,31+,32+,33+,35+/m0/s1
InChI key
XDHNQDDQEHDUTM-JQWOJBOSSA-N
General description
A macrolide antibiotic that acts as a specific inhibitor of vacuolar-type H+-ATPase (V-type; Ki = 500 pM). A valuable tool for distinguishing among different types of ATPases. Blocks lysosomal cholesterol trafficking in macrophages and is known to interfere with pH regulation in brain cells. Exhibits cytotoxic effects on a number of cell lines in a cell viability assay. Reported to selectively inhibit β-secretase, an enzyme involved in the processing of amyloid precursor protein (APP).
Note: 1 set = 3 x 10 µg
Biochem/physiol Actions
Primary Target
vacuolar type H+ ATPase
vacuolar type H+ ATPase
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 year at -20°C.
Other Notes
Sinha, S., and Lieberburg, I. 1999. Proc. Natl. Acad. Sci. USA96, 11049.
Calvert, C.M., and Sanders, D. 1995. J. Biol. Chem.270, 7272.
Nishihara, T., et al. 1995. Biochem. Biophys. Res. Commun.269, 255.
Crider, B.P., et al. 1994. J. Biol. Chem.269, 17379.
Palokangas, H., et al. 1994. J. Biol. Chem.269, 17577.
Sundquist, K.T., and Marks, S.C., Jr. 1994. J. Bone Miner. Res.9, 1575.
Drose, S., et al. 1993. Biochemistry32, 3902.
Furuchi, T., et al. 1993. J. Biol. Chem.268, 27345.
Bowman, E.J., et al. 1988. Proc. Natl. Acad. Sci. USA85, 7972.
Calvert, C.M., and Sanders, D. 1995. J. Biol. Chem.270, 7272.
Nishihara, T., et al. 1995. Biochem. Biophys. Res. Commun.269, 255.
Crider, B.P., et al. 1994. J. Biol. Chem.269, 17379.
Palokangas, H., et al. 1994. J. Biol. Chem.269, 17577.
Sundquist, K.T., and Marks, S.C., Jr. 1994. J. Bone Miner. Res.9, 1575.
Drose, S., et al. 1993. Biochemistry32, 3902.
Furuchi, T., et al. 1993. J. Biol. Chem.268, 27345.
Bowman, E.J., et al. 1988. Proc. Natl. Acad. Sci. USA85, 7972.
Disclaimer
Toxicity: Carcinogenic / Teratogenic (D)
1 of 1
This Item | ||
|---|---|---|
| assay ≥90% (HPLC) | assay ≥90% (HPLC) | assay - |
| form film | form film | form powder |
| Quality Level 100 | Quality Level 300 | Quality Level 100 |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| solubility DMSO: 100 μM, ethanol: soluble | solubility - | solubility - |
| storage condition OK to freeze, protect from light | storage condition - | storage condition OK to freeze |
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Storage Class
11 - Combustible Solids
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