189737

Streptavidin, Phycoerythrin Conjugate

This Streptavidin, Phycoerythrin Conjugate is validated for use in Enzyme Immunoassay, Flow Cytometry.

• NACRES: NA.41
• UNSPSC Code: 41116133
• Form: liquid

Properties

• biological source: (Streptomyces avidinii)
• Quality Segment: 100
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze, protect from light
• dilution: (Enzyme Immunoassay ; Flow Cytometry (1-3 µg per 1 x 10 cells in 100 µL))
• fluorescence: λ_ex 500 nm, λ_em 575 nm
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Streptavidin conjugated to R-phycoerythrin. Specific for biotin, biotinylated proteins, or biotinylated antibodies.

Application

Enzyme Immunoassay (see comments)

Flow Cytometry (1-3 µg per 1 x 10⁶ cells in 100 µl)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 50 mM sodium phosphate, 1% BSA, 0.1% glycerol, pH 7.6. Ammonium sulfate free.

Preparation Note

Do not dilute the entire vial at one time. Dilute only as needed in 50 mM sodium phosphate buffer, pH 7.2, 2-5 mg/ml of BSA or other physiological buffer.

Other Notes

Lindmo, T., et al. 1990. J. Immunol. Methods126, 183.
Starling, J.J., et al. 1988. Cancer Res.48, 6211.

The phycobiliprotein used in making this conjugate had a M.W. of 240,000. Molar extinction coefficient at 565 nm: 1.9 x 10⁶. Excitation Max: 500 nm emission max: 575 nm.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable