17-283

Rac1 Activation Assay Kit

Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody.

• Synonym(s): Activation Assay Kit, Rac1 Activity Kit, Rac1 Assay Kit
• UNSPSC Code: 12161503
• NACRES: NA.32
• eCl@ss: 32161000

Properties

• Quality Segment: 100
• species reactivity: human, rat, mouse
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable, affinity binding assay: suitable
• NCBI accession no.: NM_006908.3, NM_018890.2, NM_198829.1
• UniProt accession no.: P63000
• shipped in: dry ice
• Gene Information: human ... RAC1(5879)

Description

General description

Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Ras-related C3 botulinum toxin substrate 1 (Rac1)-GTP from cell lysates and detected by a Rac1 specific monoclonal antibody. Rac1 belongs to the Ras superfamily of small guanosine triphosphatases (GTPases). It is expressed in two major forms such as an inactive GDP-bound form and an active GTP-bound form. Rac1 is involved in influencing several physiological conditions by interacting with effectors that activate several signaling cascades.[1] The RAC1 gene is located on the human chromosome at 7p22.1.

Application

Rac1 Activation Assay Kit has been used in the detection of active Ras-related C3 botulinum toxin substrate 1 (Rac1) in brain homogenates of mice and humans by immunoblotting.[2]

Analysis Note

Routinely evaluated by precipitating Rac-GTP from 3T3 cell lysates that had been loaded with GTPγS. The precipitated Rac-GTP was detected by immunoblot analysis using anti-Rac.

Other Notes

Anti-Rac1, clone 23A8 (Cat.# 05-389)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)

Replaces: SGT425

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS05,GHS09
• signalword: Danger
• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• hcodes: H318,H410,EUH430
• pcodes: P201 - P202 - P273 - P280 - P305 + P351 + P338 - P391 - P405 - P501
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1