172104

Anaphase-Promoting Complex Inhibitor, TAME

The Anaphase-Promoting Complex Inhibitor, TAME, also referenced under CAS 1784-03-8, controls the biological activity of Anaphase-Promoting Complex. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

• Synonym(s): Anaphase-Promoting Complex Inhibitor, TAME, TAME hydrochloride, APC/C Inhibitor I, APC/C-Cdc20 Interaction Inhibitor I, Cyclosome Inhibitor I
• Empirical Formula (Hill Notation): C₁₄H₂₂N₄O₄S · xHCl
• CAS Number: 1784-03-8
• Molecular Weight: 342.41 (free base basis)
• UNSPSC Code: 12352200
• NACRES: NA.54
• MDL number: MFCD00012578
• Assay: ≥98% (HPLC)
• Form: solid
• Storage condition: OK to freeze, protect from light

Properties

• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: white
• solubility: DMSO: 50 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: [S](=O)(=O)(NC(CCC[N+H]=C(N)N)C(=O)OC)c1ccc(cc1)C.[Cl-]
• InChI: 1S/C14H22N4O4S.ClH/c1-10-5-7-11(8-6-10)23(20,21)18-12(13(19)22-2)4-3-9-17-14(15)16;/h5-8,12,18H,3-4,9H2,1-2H3,(H4,15,16,17);1H
• InChI key: JIQFFACVQXXHMY-UHFFFAOYSA-N

Description

General description

TAME is a potent ubiquitin ligase inhibitor that specifically blocks Anaphase-Promoting Complex (APC) activation by directly binding to APC and disrupts the IR-tail-dependent interactions between APC and its activator proteins Cdc20 or Cdh1. It stabilizes APC substrates and is shown to inhibit cyclin proteolysis in mitotic Xenopus egg extract with an IC₅₀ = 12 µM.

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Sackton, K.L., et al. 2014. Nature. In press.
Zeng, X., et al. 2010. Cancer Cell18, 382.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable