171536
AMPK (α1/β1/γ1), His•Tag®, Human, Recombinant, S. frugiperda
full length enzyme consisting of subunits A1, B1, and G1
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
Recommended Products
recombinant
expressed in insect cells (S. frugiperda)
Quality Level
Assay
≥95% (SDS-PAGE)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
Full length, recombinant, human AMPK subunits (A1, B1, and G1), each fused at the C-terminus to a His•Tag sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The active complex is useful in the study of kinase activity regulation and inhibitor screening.
Full length, recombinant, human AMPK subunits (A1, B1, and G1), each fused at the C-terminus to a His•Tag sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The active complex is useful in the study of kinase activity regulation and inhibitor screening. M.W. = 68,000 (A1), 38,000 (B1), 40,000 (G1).
M.W. = 68,000 (A1), 38,000 (B1), 40,000 (G1)
Warning
Toxicity: Standard Handling (A)
Physical form
In 300 mM NaCl, 150 mM Imidazole, 50 mM Sodium Phosphate, 0.2 mM DTT, 0.1 mM PMSF, 25% glycerol, pH 7.0.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Scott J.W., 2007. EMBO J26, 806.
Minokoshi Y et al. 2004. Nature428, 569.
Minokoshi Y et al. 2004. Nature428, 569.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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