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Sigma-Aldrich

Ser/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R)

Ser/Thr Phosphatase Assay Kit 1used to detect PP2A activity by either dephosphorylation of the phosphopeptide.

Synonym(s):

Phosphatase Assay Kit

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (phosphatase)

NCBI accession no.

UniProt accession no.

detection method

colorimetric

shipped in

wet ice

Gene Information

human ... PPP2R1B(5519)

Application

Ser/Thr Phosphatase Assay Kit 1used to detect PP2A activity by either dephosphorylation of the phosphopeptide.
Used to detect/quantify: Ser/Thr Kinases

Packaging

Kit capacity: 100 assays

Components

Malachite Green Solution A (Cat.# 20-105)

Malachite Green Additive (Cat.# 20-104)

Phosphate Standard (Cat.# 20-103)

Threonine Phosphopeptide (K-R-pT-I-R-R) (Cat.# 12-219)

pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)

pNPP Ser/Thr Assay Buffer (Cat.# 20-179)

NiCl2, 40mM (Cat.# 20-178)

96-well microtiter plate

Quality

routinely used to detect PP2A (Catalog # 14-111) activity by either dephosphorylation of the phosphopeptide (K-R-pT-I-R-R) or hydrolysis of pNPP

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 3 - Carc. 1A Inhalation - Met. Corr. 1 - Repr. 1B - Skin Sens. 1 - STOT RE 2

Target Organs

Lungs

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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Roumen Pankov et al.
The Journal of biological chemistry, 278(20), 18671-18681 (2003-03-15)
Integrin transmembrane receptors generate multiple signals, but how they mediate specific signaling is not clear. Here we test the hypothesis that particular sequences along the beta(1) integrin cytoplasmic domain may exist that are intimately related to specific integrin-mediated signaling pathways.
Usha Narayanan et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(52), 14349-14357 (2007-12-28)
Fragile X syndrome is a common form of inherited mental retardation and is caused by loss of fragile X mental retardation protein (FMRP), a selective RNA-binding protein that influences the translation of target messages. Here, we identify protein phosphatase 2A
Anna Signorile et al.
FEBS letters, 512(1-3), 91-94 (2002-02-20)
Immunochemical and functional evidence showing the existence in the inner membrane and matrix fraction of mammalian mitochondria of serine/threonine phosphatases acting on cAMP-dependent phosphoproteins is presented. Mg(2+)-dependent Ca(2+)-inhibitable PP2C phosphatase, associated to the inner membrane, dephosphorylates the 18 kDa (NDUFS4
K S Rutgers et al.
Neuroscience, 190, 37-42 (2011-06-21)
Previously selected amyloid beta recognizing heavy chain antibody fragments (VHH) affinity binders derived from the Camelid heavy chain antibody repertoire were tested for their propensity to cross the blood-brain barrier (BBB) using an established in vitro BBB co-culture system. Of
P Agostinis et al.
European journal of biochemistry, 205(1), 241-248 (1992-04-01)
p34cdc2 kinase, a critical regulator of the cell cycle, has been shown to recognize the consensus sequence S/TP in proteins such as histone H1, the retinoblastoma gene product RB and the carboxyl-terminal domain of eukaryotic RNA polymerase II. Using phosphorylated

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