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1.52015

Chromolith® Si (monolithic) HPLC Columns

L × I.D. 100 mm x 10 mm Column

Synonym(s):

Chromolith® SemiPrep Si 100-10 column

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Size/SKUAvailabilityPrice
1 unit
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CZK 49,100.00

About This Item

UNSPSC Code:
41115709
NACRES:
SB.52
Matrix active group:
Si, Silica bonding phase
Pore size:
13 nm mesoporosity, 2 μm macroporosity
Matrix:
monolithic silica

CZK 49,100.00


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Product Name

Chromolith® Si, L × I.D. 100 mm x 10 mm column

material

PEEK column

Quality Segment

agency

suitable for USP L3

product line

Chromolith®

feature

endcapped: no

packaging

1 ea of

parameter

150 bar pressure, 45 °C max. temp.

technique(s)

HPLC: suitable (Semi Prep HPLC), LC/MS: suitable

column L × I.D.

10 cm × 10 mm

matrix

monolithic silica

matrix active group

Si, Silica bonding phase

pore size

13 nm mesoporosity, 2 μm macroporosity

operating pH range

2-7.5

separation technique

hydrophilic interaction (HILIC), normal phase

storage temp.

no temp limit

General description

Chromolith® SemiPrep Si HPLC columns are ideally suited for direct scale-up from analytical to semiprep. This is because they offer faster sample throughput at a lower operating pressure compared to semi-prep columns packed with 5 μm particles.
Chromolith® SemiPrep 10 mm i.d. columns combine high separation speed with excellent performance. This makes them the perfect alternative to particulate columns of 10 mm i.d. (and even 21.2 mm i.d.). They have the same bimodal porous silica rod structure as Chromolith® analytical columns with an internal diameter of 4.6 mm. Their macropores are 2 μm in diameter and the mesopores are 13 nm. This combination dramatically reduces separation time while increasing efficiency.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes

The recommended Guard-columns are:
Chromolith® SemiPrep Si 10-10 guard cartridges (3 pieces) [1.52035] with the Chromolith® Guard Cartridge Holder 10 mm x 10 mm [1.52037]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Analysis Note

Pressure: ≤ 25 bar
Performance (2-nitroanisol)
Theoretical Plates (N/m) (2-nitroanisol): ≥ 80000
Capacity factor (2-nitroanisol): 1.4 - 1.9
Tailing (USP): 0.90 - 1.40 %
Selectivity
separation factor (2-nitroanisol/nitrobenzene): 3.45 - 3.95

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
1.252511.514651.52035
matrix active group

Silica bonding phase

matrix active group

Silica bonding phase

matrix active group

Silica bonding phase

matrix active group

Silica bonding phase

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

technique(s)

HPLC: suitable (Semi Prep HPLC), LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable (Prep LC), UHPLC-MS: suitable, UHPLC: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable (Semi Prep HPLC), LC/MS: suitable

agency

suitable for USP L3

agency

suitable for USP L3

agency

suitable for USP L3

agency

suitable for USP L3

pore size

13 nm mesoporosity, 2 μm macroporosity

pore size

12 nm mesoporosity, 3 μm macroporosity

pore size

13 nm mesoporosity, 2 μm macroporosity

pore size

13 nm mesoporosity, 2 μm macroporosity

product line

Chromolith®

product line

Chromolith®

product line

Chromolith®

product line

Chromolith®


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