14-736-M

TAO2 Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, TAO2. TAO2 is a MAP3K that doubly phosphorylates & activates the MAP2Ks, MEK3 & MEK6. For use in Kinase Assays.

• UNSPSC Code: 12352202
• NACRES: NA.32
• eCl@ss: 32160405
• Assay: 84% (Coomassie blue staining, SDS-PAGE)
• Biological source: human
• Recombinant: expressed in Sf21 cells
• Mol wt: M_w 138.3 kDa

Properties

• biological source: human
• Quality Segment: 100
• recombinant: expressed in Sf21 cells
• assay: 84% (Coomassie blue staining, SDS-PAGE)
• specific activity: 1562 U/mg
• mol wt: M_w 138.3 kDa
• manufacturer/tradename: Chemicon^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_004783.2, NM_016151.2
• UniProt accession no.: Q9UL54
• Gene Information: human ... TAOK2(9344)

Description

General description

N-terminal 6His-tagged, recombinant, TAO2. TAO2 is a mitogen-activated protein kinase kinase kinase (MAP3K) that doubly phosphorylates and activates the MAP kinase kinases (MAP2Ks) MEK3 and MEK6.

Product Source: expressed in Sf21 cells

Application

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Biochem/physiol Actions

Protein Target: TAO2

Target Sub-Family: STE

Physical form

10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Ni²⁺/NTA-agarose

Preparation Note

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Analysis Note

Routinely evaluated by phosphorylation of 0.8 mg/ml myelin basic protein (MBP). Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of TAO2.

Other Notes

Specific Activity: where one unit of TOA2 activity is defined as 1 nmol phosphate incorporated into 0.8 mg/mL myelin basic per minute at 30°C with a final ATP concentration of 100 μM.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable