12-301

Non-Stimulated A431 Cell Lysate

• UNSPSC Code: 41105330
• NACRES: NA.41
• eCl@ss: 32011807
• Technique(s): western blot: suitable
• Species reactivity: -
• Application: WB
• Citations: 7

Properties

• biological source: human
• Quality Segment: 100
• form: liquid
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• shipped in: dry ice

Description

Biochem/physiol Actions

A431

Physical form

modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM NaCl, 1mM EDTA, 1mM PMSF, 1μg/ml each of aprotinin, leupeptin, pepstatin, 1mM Na₃VO₄, 1mM NaF) and diluted with non-reducing sample buffer (31mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue).

Preparation Note

1 year at -20°C

Analysis Note

Routinely evaluated as a positive control for several of Upstates antibodies.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS07
• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• signalword: Danger
• hcodes: H319,H412,EUH430
• pcodes: P201 - P202 - P273 - P391 - P405 - P501
• Hazard Classifications: Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2