1.09211

Azure II (C.I.52010/52015)

for microscopy

• Synonym(s): Azure II, 3,7 Bis(dimethylamino)phenothiazin-5-ium chloride and 3-(Dimethylamino)-7-(methylamino)-phenothiazin-5-ium chloride
• Linear Formula: C₁₆H₁₈N₃S · C₁₅H₁₆N₃S ·2 Cl
• CAS Number: 37247-10-2
• Molecular Weight: 625.68
• MDL number: MFCD00080716
• UNSPSC Code: 41116130
• NACRES: NA.21
• Colour Index Number: 52010/52015

Properties

• Quality Segment: 400
• form: solid
• IVD: for in vitro diagnostic use
• pH: 2.9 (20 °C, 10 g/L in H₂O)
• solubility: 100 g/L
• bulk density: 610 kg/m³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• SMILES string: [s+]1c2c(nc3c1cc(cc3)N(CC)CC)ccc(c2)N(CC)CC, [Cl-].[Cl-].CNc1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](\C)C.CN(C)c4ccc5N=C6C=C\C(C=C6Sc5c4)=[N+](\C)C
• InChI: 1S/C16H18N3S.C15H15N3S.2ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;;/h5-10H,1-4H3;4-9H,1-3H3;2*1H/q+1;;;/p-1
• InChI key: YOWZJZJLXUQHGF-UHFFFAOYSA-M

Description

General description

Azure II (C.I. 52010/ 52015), is a dry dye for the preparation of a color staining solution used for the staining of blood and bone marrow smears of human origin. The present dye Azure II (C.I.52010/52015) - for microscopy is a defined dye mixture of azure I and methylene blue (1+1), for use in hematological stainings by addition of eosin Y. The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y.
Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• pcodes: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3