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1.06177

Millipore

TBE buffer

10 x pH 8.3 tris-borate-EDTA buffer

Synonym(s):

10X TBE running buffer, Tris-Borate EDTA buffer

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

form

liquid

pH

8.2-8.4 (20 °C in H2O)

density

1.06 g/cm3 at 20 °C

storage temp.

15-25°C

General description

Tris, borate, and ethylenediaminetetraacetic (TBE) buffer is routinely used to conduct DNA and RNA agarose gel electrophoresis. It comprises a weak acid in neutral and anionic forms (e.g., COOH and COO- species) and a weak base, Tris, which is either in neutral or cationic forms (Tris-NH2 and Tris-NH3+). These ions buffer the pH, maintain a low conductivity medium, and transfer the electrical current. Ethylenediaminetetraacetic (EDTA) is used to chelate Mg2+ ions and inactivate the potential DNA nucleases. TBE is also used to perform a good resolution of DNA fragments that slightly enhances the separation of smaller fragments. It also protects nucleic acids from enzymatic degradation.

Analysis Note

Appearance (colour): almost colourless
Appearance (description): almost clear
pH-value: 8.2 - 8.4

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H317 - H360FD - H412

Hazard Classifications

Aquatic Chronic 3 - Repr. 1B - Skin Sens. 1

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Optical properties of buffers and cell culture media for optofluidic and sensing applications
Hoang VT, et al.
Applied Sciences, 9(6), 1145-1145 (2019)
Brian A Sanderson et al.
Analytical biochemistry, 454, 44-52 (2014-03-19)
Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric

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