Precoated Kieselguhr or mixed Silica gel/Kieselguhr plates are only of small importance nowadays. Kieselguhr layers are used like cellulose layers for the TLC separation of polar and moderately polar substances. Polyamide, on the other hand , is used for the separation of flavonoids and other phenolic substances.
Linkage
Replaces: 5738-7; 5738
Analysis Note
Layer thickness: 180 - 220 µm Deviation of layer thickness per plate: ≤ 35 µm Chromatographic testing: amino acid test hRf-values - β-alanine, short-time test: 20 - 30 - L-alanine, amino acid test: 40 - 50 - DL-Valin, amino acid test: 75 - 85 - L-leucine, amino acid test: 88 - 95 Typical value determined on a plate Eluent: n- propanol/ water (80/20 v/v)
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