1.04193

Auramine O (C.I. 41000)

• Empirical Formula (Hill Notation): C₁₇H₂₂ClN₃
• CAS Number: 2465-27-2
• Molecular Weight: 303.83
• NACRES: NA.21
• UNSPSC Code: 12352116
• MDL number: MFCD00012484

Properties

• form: solid
• Quality Segment: 200
• potency: 1000 mg/kg LD₅₀, oral (Rat)
• pH: 6-7 (20 °C, 10 g/L in H₂O)
• mp: 265 °C (decomposition)
• solubility: 10 g/L
• bulk density: 430 kg/m³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
• InChI key: KSCQDDRPFHTIRL-UHFFFAOYSA-N

Description

General description

Auramine O (C.I. 41000)-for microscopy, is a dry dye for fluorescence and can be used acc. to Hagemann-Hermannof for the staining acid-fast bacteria (AFB) in smears and sections of human origin . The mechanism of the fluorescence staining is similar to the classical Ziehl-Neelsen method. The cell wall of AFB has a high proportion of wax and lipids and hence absorbs dyes only very slowly. Once the AFB have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, AFB are termed as acid- and alcohol-fast for staining. Pretreatment of the specimens with Sputofluol^™ dissolves the bacteria from the surrounding viscid sputum and cell material. Sputofluol^™ also has a disinfectant effect, with the result that any microorganisms that are present are killed off. The dye has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.

Application

for microscopy

Analysis Note

Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (buffer pH 6.0)431 - 433 nmSpec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; buffer pH 6.0; calc. on dried substance)1100 - 1300TLC-Testpasses testLoss on drying (110 °C)≤ 5 %Suitability for microscopypasses test

Legal Information

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08,GHS07,GHS09
• Signal Word: Warning
• Hazard Codes: H302,H319,H351,H411
• Precautionary Statements: P202 - P264 - P273 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2
• Storage Class: 11 - Combustible Solids
• WGK: WGK 3