1.04178

Rhodamine B (C.I.45170)

• Empirical Formula (Hill Notation): C₂₈H₃₁ClN₂O₃
• CAS Number: 81-88-9
• Molecular Weight: 479.01
• NACRES: NA.21
• UNSPSC Code: 12352116
• MDL number: MFCD00011931

Properties

• form: solid
• Quality Segment: 200
• potency: >2000 mg/kg LD₅₀, oral (Rat)
• composition: Dye, ≥90% spectrophotometric assay
• pH: 3-4 (20 °C, 10 g/L in H₂O)
• mp: 199-201 °C
• solubility: 15 g/L
• density: 1.31 g/cm³ at 20 °C
• bulk density: 250 kg/m³
• application(s): diagnostic assay manufacturing; hematology; histology; microbiology
• storage temp.: 2-30°C
• InChI: 1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H
• InChI key: PYWVYCXTNDRMGF-UHFFFAOYSA-N

Description

General description

Rhodamine B (C.I. 45170) for microscopy, is a solid dye for fluorescence AFB staining of bacteriological smears and histological tissue sections of human origin. The mechanism is a non-specific binding of fluorescence dye on a substrate, similar to the classical Ziehl-Neelsen method, although the heating of the Rhodamine B color solution can be avoided. The dye has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.

Application

for microscopy

Analysis Note

Dye content (spectrophotometrically): ≥ 90 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 550 - 552 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 2115 - 2350
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

Safety Information

• pictograms: GHS05,GHS07
• signalword: Danger
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• hcodes: H302,H318,H412
• pcodes: P264 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P501