1.04178
Rhodamine B (C.I.45170)
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About This Item
Empirical Formula (Hill Notation):
C28H31ClN2O3
CAS Number:
Molecular Weight:
479.01
NACRES:
NA.21
UNSPSC Code:
12352116
MDL number:
form
solid
Quality Segment
potency
>2000 mg/kg LD50, oral (Rat)
composition
Dye, ≥90% spectrophotometric assay
pH
3-4 (20 °C, 10 g/L in H2O)
mp
199-201 °C
solubility
15 g/L
density
1.31 g/cm3 at 20 °C
bulk density
250 kg/m3
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-30°C
InChI
1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H
InChI key
PYWVYCXTNDRMGF-UHFFFAOYSA-N
General description
Rhodamine B (C.I. 45170) for microscopy, is a solid dye for fluorescence AFB staining of bacteriological smears and histological tissue sections of human origin. The mechanism is a non-specific binding of fluorescence dye on a substrate, similar to the classical Ziehl-Neelsen method, although the heating of the Rhodamine B color solution can be avoided. The dye has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.
Application
for microscopy
Analysis Note
Dye content (spectrophotometrically): ≥ 90 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 550 - 552 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 2115 - 2350
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 550 - 552 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 2115 - 2350
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
hcodes
Certificates of Analysis (COA)
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