1.00439

ChromoCult^® Listeria Agar Enrichment-Supplement

ChromoCult^®, selective for Listeria spp.

• Synonym(s): Listeria Enrichment Supplement
• NACRES: NA.74
• UNSPSC Code: 41106212

Properties

• Quality Segment: 100
• agency: APHA, ISO 11133, ISO 11290-1, ISO 11290-2
• form: lyophilized powder
• manufacturer/tradename: ChromoCult^®
• technique(s): microbiological culture: suitable
• pH: 6.6 (20 °C in H₂O)
• application(s): food and beverages; microbiology; pathogen testing
• compatibility: for use with ChromoCult^® Listeria agar (base) acc. OTTAVIANI and AGOSTI (1.00427)
• storage temp.: 2-8°C
• suitability: selective for Listeria spp. (Agar)

Description

General description

ChromoCult^® Listeria Agar Enrichment-Supplement is a homogeneous, sterile suspension of L-α-phosphatidylinositol in distilled water. Differentiation of Listeria monocytogenes from non-pathogenic Listeria sp. is achieved through the production of a phosphatidylinositol-specific phospholipase C (PI-PLC). Listeria monocytogenes hydrolyses the specific purified substrate added to the medium producing an opaque halo around the colonies.

Application

ChromoCult^® Listeria Agar Enrichment-Supplement is used as an additive for the preparation of ChromoCult^® Listeria Selective Agar (1.00427) for detection and enumeration of Listeria monocytogenes and of Listeria sp. in a variety of food products.

Analysis Note

Growth promotion test according to ISO 11133.
Inoculum on reference medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
10 - 100
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100
- Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100
Recovery on test medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
≥ 70 %
- Listeria monocytogenes ATCC 19111 (WDCM 00020): ≥ 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %
Colonies
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
blue-green; non-transparent zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone
- Listeria innocua ATCC 33090 (WDCM 00017): blue-green
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): no growth
Incubation: Listeria 24-48 Std. 37°C aerobic; other strains at least 48 hrs.

Other Notes

Replaces: 1004390017

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable