09-456

Anti-AIRE Antibody

Upstate^®, from rabbit

• Synonym(s): APECED protein, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy ; ; protein, autoimmune polyendocrinopathy candidiasis ectodermal dystrophy 2 ; ; autoimmune regulator ; ; , autoimmune regulator (APECED protein), autoimmune
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 16

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_000383.2, NM_000658.1
• UniProt accession no.: O43918
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Aire(11634)

Description

General description

65 kDa

AIRE (Autoimmune Regulator) is a human gene that is expressed primarily in the thymus. It causes transcription of a wide selection of organ-specific genes, reducing the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells via negative selection. Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendo-crinopathy-candidiasis-ectodermal dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. Aire functions through initiating the transcription of a diverse set of self-antigens, such as insulin, in the thymus. This expression then allows maturing thymocytes to become tolerant towards peripheral organs, thereby suppressing autoimmune disease. Aire is atranscriptional regulator protein that binds to DNA as dimer and tetramer, but not as a monomer. It forms nuclear bodies and interacts with the transcriptional coactivator CBP (CREBBP). At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD).

Immunogen

Epitope: a.a. 162-181

KLH-conjugated synthetic peptide corresponding to amino acids 162-181 of mouse AIRE

Application

Suggested dilutions:
Western blot: 1:1000

Detect AIRE using this Anti-AIRE Antibody validated for use in WB.

Research Category
Inflammation & Immunology

Research Sub Category
Developmental Signaling

Transcription Factors

RNA Binding Protein (RBP)

Biochem/physiol Actions

Likely to cross-react with rat, based on sequence homology. Cross-reactivity with other species has not been tested

Recognizes murine AIRE, Mr 58 kDa (calculated). Appears at ~65 kDa in Western blots.

Physical form

100 μg of immunoaffinity purified rabbit polyclonal IgG in 173 μl of 0.02M PBS, 0.25M NaCl containing 0.1% sodium azide

ImmunoAffinity Purified

Preparation Note

2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Analysis Note

Control
Murine thymus tissue lysate

Routinely evaluated by Western Blot analysis.

Western Blot:
A 1:1000 dilution of this lot detected AIRE in murine thymus lysate

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• wgk: WGK 2
• Storage Class: 12 - Non Combustible Liquids