07-877

Anti-phospho-PKCη (Thr655) Antibody

Upstate^®, from rabbit

• Synonym(s): PRKCH, PKCeta, PKCh, protein kinase C, eta, KPCL
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human
• Application: WB
• Citations: 2

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_006255.3
• UniProt accession no.: P24723
• shipped in: dry ice
• target post-translational modification: phosphorylation (pThr655)
• Gene Information: human ... PRKCH(5583)

Description

General description

~80 kDa

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-PKCη (Thr655).

Application

Immunoblot Analysis: A 0.50 μg/mL dilution of this lot detected phospho-PKCη (Thr655) in RIPA lysate of PMA treated Jurkat cells.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

This Anti-phospho-PKCη (Thr655) Antibody is validated for use in WB for the detection of phospho-PKCη (Thr655).

Biochem/physiol Actions

Predicted to cross-react with Mouse and Rat (100% homologous) based on sequence homology.

Recognizes phosphorylated PKCη (Thr655). Not expected to cross-react with other PKC isoforms

Physical form

100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide).

Affinity purified

Preparation Note

Stable for 2 years at -80°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Routinely evaluated by immunoblot.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable