07-452

Anti-dimethyl-Histone H3 (Lys27) Antibody

Upstate^®, from rabbit

• Synonym(s): H3K27me2, Histone H3 (di methyl K27)
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human
• Application: ChIP, DB, WB, cell based assay, multiplexing
• Citations: 146

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable (ChIP-seq), cell based assay: suitable, dot blot: suitable, multiplexing: suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_003493
• UniProt accession no.: Q16695
• shipped in: wet ice
• target post-translational modification: dimethylation (Lys27)
• Gene Information: human ... H3C1(8350)

Description

General description

17 kDa

Anti-dimethyl-Histone H3 (Lys27)

Immunogen

KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...AR_me2KSA... in which _me2K corresponds to dimethyl-lysine at residue 27 of human histone H3

Application

Additional Referenced Research Applications:
Dot Blot, Immunocytochemistry (cells)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-dimethyl-Histone H3 (Lys27) Antibody (rabbit polyclonal antibody) validated in Mplex, PIA, WB, ChIP-seq, DB to detect dimethyl-Histone H3 (Lys27) also known as H3K27me2, Histone H3 (di methyl K27).

Biochem/physiol Actions

Broad species cross-reactivity expected, based on sequence homology

Recognizes dimethylated histone H3 (Lys27).

Physical form

Affinity purified IgG in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% NaN3.

Peptide affinity purified

Preparation Note

Stable for 2 years at 2-8°C from date of shipment.

Analysis Note

Control
HeLa whole cell lysate, U2OS cell lysate

Routinely evaluated by immunoblot on acid extracted proteins from HeLa cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable