05-484

Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3

clone 20G3, Upstate^®, from mouse

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 20G3, monoclonal
• Species reactivity: human, mouse
• Application: IP, WB
• Citations: 7

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 20G3, monoclonal
• species reactivity: human, mouse
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NM_005228
• UniProt accession no.: P00533
• shipped in: wet ice
• target post-translational modification: phosphorylation (non-phospho-Tyr1173)
• Gene Information: human ... EGFR(1956) ; mouse ... Egfr(13649)

Description

General description

175kDa

Immunogen

peptide containing the unphosphorylated Tyr1173 motif (NAE[Y]LR) of the human EGF receptor

Application

Detect EGFR (non-phospho-Tyr1173) using this Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3 validated for use in IP & WB.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Biochem/physiol Actions

The motif NAEYLR of the EGF-R (unphosphorylated at Tyr1173), this antibody does not interact with the phosphorylated (pTyr1173) form of the EGF receptor. Tyr1173 corresponds to Tyr1197 in the human (isoform a) and mouse precursor proteins, and to Tyr1196 in the mature rat and pig precursor proteins.

Physical form

100µg of lyophilized thiophilic and size exclusion chromatography purified mouse IgG1 from 1mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.

Format: Purified

Thiophilic adsoption and size exclusion chromatography

Preparation Note

2 years at -20°C

Analysis Note

routinely evaluated by immunoblot on RIPA lysates from unstimulated HFF cells

Other Notes

Replaces: 04-337; 04-341

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1