05-440

Anti-Cbl Antibody, clone 7G10

clone 7G10, Upstate^®, from mouse

• Synonym(s): Cas-Br-M (murine) ecotropic retroviral transforming sequence, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-CBL, RING finger protein 55, Signal transduction protein CBL, oncogene CBL2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 7G10, monoclonal
• Application: ICC, IP, WB
• Citations: 14

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 7G10, monoclonal
• species reactivity: mouse, rat, human
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NM_005188
• UniProt accession no.: P22681
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CBL(867)

Description

General description

120kDa

Casitas B-lineage lymphoma (Cbl), is an oncogene which causes pre B-cell lymphomas. It participates in the regulation of cell signaling and has the ability to interact with many proteins such as receptor/non-receptor PTK′s, structural proteins, and novel proteins that are not well understood. Diseases associated with Cbl are autoimmune diseases, diabetes, and leukemia. By modifying Cbl′s ability to bind to oncogenic proteins, Cbl may be developed into an anti-cancer tool and in the near future used in therapeutic intervention.

Immunogen

Epitope: a.a. 878-906

GST fusion protein corresponding to residues 878-906 of human Cbl

Application

Immunocytochemistry:
5-10 μg/mL of a previous lot showed positive immunostaining for Cbl in 3T3/A31 cells fixed with ice cold Methanol/Ethanol [1:1] for 1 minute.

Immunoprecipitation:
2 μg of a previous lot immunoprecipitated Cbl from 1 mg of a mouse 3T3/A31 cell RIPA lysate, as reported by an independent laboratory.

Research Category
Protein Trafficking

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

This Anti-Cbl Antibody, clone 7G10 is validated for use in IP, WB, IC for the detection of Cbl.

Biochem/physiol Actions

Antibody recognizes amino acids 878-906 on human Cbl

Physical form

Format: Purified

Protein G Chromatography

Purified IgG1k in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4

Preparation Note

Stable for 1 year at 2-8ºC from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

routinely evaluated by immunoblot on RIPA lysates from human Jurkat, mouse 3T3/A31, TF-1, or FDC-P1/Mac11

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1