05-1075

Anti-FOXO1 Antibody, clone 2H8.2

clone 2H8.2, from mouse

• Synonym(s): Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, forkhead box O1, forkhead box O1A (rhabdomyosarcoma), forkhead homolog in rhabdomyosarcoma
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 2H8.2, monoclonal
• Application: ICC, IHC, WB
• Citations: 9

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2H8.2, monoclonal
• species reactivity: rat, human, mouse
• packaging: antibody small pack of 25 μg
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_002006.2
• UniProt accession no.: Q12778
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... FOXO1(2308) ; mouse ... Foxo1(56458)

Description

General description

Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. (Gross, D.N., 2009).

~78-82 kDa

Immunogen

GST-tagged recombinant protein corresponding to full length FOXO1.

Application

Immunocytochemistry: A 1:500 dilution of a previous lot was used.

Immunocytochemistry: Representative lot data.
A 1:500 dilution of a previous lot was used.

Immunohistochemistry (Paraffin): Representative lot data.
Optimal Staining With Epitope Retrieval: Ductal Carcinoma In Situ.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-FOXO1 Antibody, clone 2H8.2 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect FOXO1 also known as Forkhead box protein O1A, Forkhead in rhabdomyosarcoma.

Biochem/physiol Actions

This antibody detects FOXO1.
FOXO1 shuttles between the nucleus and the cytosol.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
HEK293 cell lysate

Evaluated by western blot on HEK293 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected FOXO1 in HEK293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1005

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable