04-841

Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal

culture supernatant, clone A9W4, from rabbit

• Synonym(s): RhoA-binding kinase 2, Rho-associated, coiled-coil containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, p150 ROK-alpha, p164 ROCK-2, ROCK-II, ROKalpha, Rock2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: A9W4, monoclonal
• Application: IP, WB
• Citations: 6

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: A9W4, monoclonal
• species reactivity: rat, human, mouse, bovine
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NM_013022
• UniProt accession no.: Q62868
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: rat ... Rock2(25537)

Description

General description

150 kDa

Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.

Immunogen

6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II.

Application

Detect ROKα/ROCK-II using this Anti-ROKα/ROCK-II Antibody, clone A9W4 validated for use in IP & WB.

Immunoprecipitation:
4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Biochem/physiol Actions

Predicted to cross-react with mouse and bovine based on sequence homology.

Recognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa.

Physical form

Cultured supernatant containing 0.05% sodium azide.

Preparation Note

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
RIPA lysates from PC-12 cells

Routinely evaluated by Western BLot on RIPA lysates from PC-12 cells.

Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.

Other Notes

Replaces: 05-841

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1