04-822
Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal
culture supernatant, clone 3L74, Upstate®
Synonym(s):
ras homolog
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 μL | Estimated to ship onMarch 25, 2026fromAreál Kühne+Nagel spol. s r.o. | CZK 11,000.00 |
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3L74, monoclonal
Application:
WB
Citations:
12
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
3L74, monoclonal
species reactivity
rat, human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
Looking for similar products? Visit Product Comparison Guide
1 of 1
This Item | |||
|---|---|---|---|
| clone 3L74, monoclonal | clone 3L74, monoclonal | clone 55, monoclonal | clone 4, monoclonal |
| species reactivity rat, human | species reactivity rat, human | species reactivity human, mouse, rat | species reactivity mouse, human |
| Gene Information human ... RHOA(387) | Gene Information human ... RHOA(387) | Gene Information human ... RHOA(387) | Gene Information human ... RND3(390) |
| biological source rabbit | biological source rabbit | biological source mouse | biological source mouse |
| antibody form culture supernatant | antibody form purified antibody | antibody form purified immunoglobulin | antibody form ascites fluid |
| isotype IgG | isotype IgG | isotype IgG1 | isotype IgG |
General description
Mr 22kDa
Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganisation, and membrane trafficking.
Immunogen
KLH-conjugated, synthetic peptide (SYPDTDVILMCFSIDSPDSLENKK) corresponding to amino acids 73-94 of human Rho (-A, -B, -C), with two terminal lysines added to increase peptide solubility
Application
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
This Anti-Rho (-A Antibody, -B, -C), clone 3L74 is validated for use in WB for the detection of Rho (-A, -B, -C).
Western Blot Analysis:
A 1:1000-1:4000 dilution of this lot detected Rho (-A, -B, -C) in RIPA lysates from human HL-60 and rat PC-12 cells. This antibody also recognized recombinant RhoA protein, but not recombinant rac1 or cdc42 proteins.
A 1:1000-1:4000 dilution of this lot detected Rho (-A, -B, -C) in RIPA lysates from human HL-60 and rat PC-12 cells. This antibody also recognized recombinant RhoA protein, but not recombinant rac1 or cdc42 proteins.
Biochem/physiol Actions
Broad species cross-reactivity expected based on sequence homology
Recognizes Rho (-A, -B, -C)
Physical form
Cultured supernantant in 0.05% sodium azide
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
routinely evaluated by immunoblot on modified RIPA lysates from HL-60 cells
Other Notes
Replaces: 05-822
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Yukie Yamahashi et al.
The Journal of biological chemistry, 286(52), 44576-44584 (2011-11-11)
Partitioning-defective 1b (PAR1b), also known as microtubule affinity-regulating kinase 2 (MARK2), is a member of evolutionally conserved PAR1/MARK serine/threonine kinase family, which plays a key role in the establishment and maintenance of cell polarity at least partly by phosphorylating microtubule-associated
Aurora M Fontainhas et al.
Investigative ophthalmology & visual science, 49(9), 4177-4187 (2008-05-27)
Adult salamander photoreceptors retract existing axons and extend new neuritic processes in vitro. In mammalian retina, similar forms of structural plasticity occur in injury and disease. The authors asked whether RhoA is present in photoreceptor axon terminals and whether activity
Tsubasa Tanaka et al.
Development (Cambridge, England), 138(12), 2523-2532 (2011-05-26)
Drosophila pole (germ) plasm contains germline and abdominal determinants. Its assembly begins with the localization and translation of oskar (osk) RNA at the oocyte posterior, to which the pole plasm must be restricted for proper embryonic development. Osk stimulates endocytosis
Alistair Rice et al.
Cancers, 12(2) (2020-01-30)
The invasive properties of cancer cells are intimately linked to their mechanical phenotype, which can be regulated by intracellular biochemical signalling. Cell contractility, induced by mechanotransduction of a stiff fibrotic matrix, and the epithelial-mesenchymal transition (EMT) promote invasion. Metastasis involves
Prognostic value of rho GTPases and rho guanine nucleotide dissociation inhibitors in human breast cancers.
Jiang, Wen G, et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 9, 6432-6440 (2003)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 04-822 | 04053252277870 |
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service