04-742

Anti-SHP-1/2 Antibody, clone NL213, rabbit monoclonal

culture supernatant, clone NL213, Upstate^®

• Synonym(s): Anti-DRPLA
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: NL213, monoclonal
• Application: IP, WB
• Citations: 2

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: NL213, monoclonal
• species reactivity: human, mouse
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_002831, NM_002834
• UniProt accession no.: P29350
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... PTPN6(5777) ; mouse ... Ptpn6(15170)

Description

General description

~65kDa

Immunogen

Full-length glutathione transferase SHP-1 fusion protein, purified from bacteria

Application

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

This Anti-SHP-1/2 Antibody, clone NL213 is validated for use in IP, WB for the detection of SHP-1/2.

Biochem/physiol Actions

Others not tested.

Recognizes SHP-1 greater than SHP-2, Mr ~65 kDa

Physical form

Cultured supernantant in 0.05% sodium azide

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

routinely evaluated by immunoblot in RIPA lysates from human A431 carcinoma cells

Other Notes

Replaces: 05-742

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable