4′,5′-Dibromofluorescein (DBF) is a fluorone dye. Fluorone dyes are hydroxyl-xanthene dyes (homologues of fluorescein) and have been the most widely used class of organic fluorophores for labeling and sensing biomolecules. DBF is routinely used as a ligand for studying the properties of protein using spectral analysis.
Application
4′,5′-Dibromofluorescein has been used to stain proteins in 1-D and 2-D sodium dodecyl sulfatepolyacrylamide gel electrophoresis SDS-PAGE. This method is rapid, economical, and highly sensitive in comparison with other proteomic research staining methods.
European journal of drug metabolism and pharmacokinetics, 45(6), 809-815 (2020-07-15)
Citrus flavonoids are not only components of daily nutrition, they are also promoted as dietary supplements and are important ingredients in traditional medicines. Interactions of flavonoids with synthetic drugs represent an often neglected issue. We therefore investigated in vitro whether
Journal of biochemical and molecular toxicology, 33(10), e22379-e22379 (2019-08-01)
The interactions of six neonicotinoid pesticides and one neonicotinoid metabolite with drug transporters have been characterized in vitro. Acetamiprid, clothianidin, imidacloprid, nitenpyram, thiacloprid and its metabolite thiacloprid amide, and thiamethoxam, each used at 100 µM, did not impair activity of the
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