200220

Azure II eosinate

powder

• Synonym(s): Azure II-eosin; Methylene blue, eosinate of Azure II
• CAS Number: 53092-85-6
• NACRES: NA.47
• PubChem Substance ID: 24851351
• UNSPSC Code: 12171500
• MDL number: MFCD00084753

Properties

• form: powder
• Quality Segment: 100
• technique(s): microbe id | staining: suitable
• color: dark green
• solubility: soluble, hazy to turbid
• Amax: ≥0.50 at 644-655 nm in methanol at 0.005 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[Cl-].[Cl-].CNc1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C.CN(C)c4ccc5N=C6C=C\C(C=C6Sc5c4)=[N+](/C)C.[O-]C(=O)c7ccccc7C8=C9C=C(Br)C(=O)C(Br)=C9Oc%10c(Br)c([O-])c(Br)cc8%10
• InChI: 1S/C20H8Br4O5.C16H18N3S.C15H15N3S.2ClH.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;;;;/h1-6,25H,(H,27,28);5-10H,1-4H3;4-9H,1-3H3;2*1H;;/q;+1;;;;2*+1/p-3
• InChI key: CSDDIABEPMTRIP-UHFFFAOYSA-K

Description

General description

Azure II Eosinate is the eosinate of the dye Azure II which is a mixture of the dyes methylene blue and azure B in equal proportions. This compound is a key ingredient in Giemsa stain, which is widely utilized for staining blood elements and facilitating the visualization of cellular components under a microscope.

Application

Azure II Eosinate is used for human-medical cell staining and serves the purpose of the investigation of sample material of human origin (by fixing, embedding where necessary, staining, counterstaining, mounting) in hematological and clinico-cytological specimen materials, for example smears of whole blood and bone marrow.

Biochem/physiol Actions

The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azure B with DNA. Both dyes assemble to an Eosin Y- Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances.

Features and Benefits

Chief ingredient of Giemsa Stain.

Safety Information

• pictograms: GHS05
• signalword: Danger
• hcodes: H318
• pcodes: P280 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves