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Key Documents

23362-U

Supelco

SP®-2560 Capillary GC Column

L × I.D. 100 m × 0.25 mm, df 0.20 μm

Synonyma:

F.S. Cap. SP-2560 100m 0.25mm 0.20um, 5inch cage

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About This Item

UNSPSC Code:
12352211
eCl@ss:
32119290
NACRES:
SB.54

material

fused silica

agency

AOAC® 2012.13,Ce 1-h05,996.06
meets requirements for USP G5

parameter

≤25-250 °C temperature (isothermal or programmed)

Beta value

313

df

0.20 μm

technique(s)

gas chromatography (GC): suitable

L × I.D.

100 m × 0.25 mm

matrix active group

Non-bonded; poly(biscyanopropyl siloxane) phase

application(s)

agriculture
clinical
food and beverages

column type

capillary highly polar

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General description

P/N 23362-U is wound on a 5" cage designed to fit an Agilent 6850 GC.
Application: This highly polar biscyanopropyl column was specifically designed for detailed separation of geometricpositional (cis/trans) isomers of fatty acid methyl esters (FAMEs). It is extremely effective for FAME isomer applications.
USP Code: This column meets USP G5 requirements.
Phase:
  • Non-bonded
  • Poly(biscyanopropyl siloxane)
Temp. Limits:
  • Subambient to 250 °C (isothermal or programmed)

Application

SP®-2560 capillary GC column may be used for analysing fatty acid methyl esters (FAME) using capillary gas liquid chromatography (GLC).

Other Notes

We offer a variety of chromatography accessories including analytical syringes

Legal Information

AOAC is a registered trademark of AOAC International
SP is a registered trademark of Sigma-Aldrich Co. LLC

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Determination ofcis andtrans-Octadecenoic acids in margarines by gas liquid chromatography-infrared spectrophotometry.
Ratnayake, W. M. N., et al.
Journal of the American Oil Chemists' Society, 67, 804-810 (1990)
Pierluigi Delmonte et al.
Journal of chromatography. A, 1218(3), 545-554 (2010-12-24)
The ionic liquid SLB-IL111 column, available from Supelco Inc., is a novel fused capillary gas chromatography (GC) column capable of providing enhanced separations of fatty acid methyl esters (FAMEs) compared to the highly polar cyanopropyl siloxane columns currently recommended for
W M Nimal Ratnayake et al.
Journal of AOAC International, 85(5), 1112-1118 (2002-10-11)
This study examines the effect of the column operating temperature of 100 m SP-2560 and CP-Sil 88 capillary gas chromatographic (GC) columns on the separation of cis- and trans-octadecenoic (18:1) isomers in partially hydrogenated vegetable oils. The overlapping GC peaks
W M Ratnayake
Journal of AOAC International, 78(3), 783-802 (1995-05-01)
An infrared spectrophotometric (IR) method for the determination of total trans unsaturated fatty acid (trans) content and a combined gas-liquid chromatographic/infrared spectrophotometric (GC/IR) method for determination of fatty acid composition of partially hydrogenated vegetable oils (PHVO) were studied collaboratively in
Noelia Aldai et al.
Lipids, 47(8), 821-825 (2012-06-15)
Rumen metabolism (e.g., biohydrogenation) of dietary unsaturated fatty acids (FA) is one of the main reasons why ruminant fats tend to be highly saturated and contain many isomerized FA intermediates. The process by which long-chain (20- to 24-carbon FA) polyunsaturated

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