T6884

Thrombin from human plasma

lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280, 18.3)

• Synonyma: Factor IIa
• Číslo CAS: 9002-04-4
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-648-7
• MDL number: MFCD00082072
• Číslo EC: 3.4.21.5 (BRENDA, IUBMB)
• Specific activity: ≥2,000 NIH units/mg protein (E1%/280, 18.3)
• Biological source: human plasma

Vlastnosti

• biological source: human plasma
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥2,000 NIH units/mg protein (E1%/280, 18.3)
• mol wt: 37.4 kDa
• impurities: HIV and HBsAg, source material tested negative
• UniProt accession no.: P00734
• storage temp.: −20°C
• Gene Information: human ... F2(2147)

Popis

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Thrombin is produced from the proteolytic cleavage of inactive prothrombin in the liver. It is a glycoprotein with four structural domains: the 10 γ-carboxylated glutamic acid containing Gal domain, two kringle domains, and the trypsin-like serine protease domain. The prothrombin gene is mapped to human chromosome 11p11.2.

Application

Thrombin from human plasma has been used:

• in the preparation of fibrin gels for assessing human bone marrow stromal cells (hBMSC) morphology,
• in the activation of platelets,
• to evaluate the integrity of endothelial cell (EC) monolayers

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags.

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to investigate the protein C pathway in intestinal barrier function.

The main function of thrombin is the cleavage of fibrinogen to fibrin, to assist stable clot formation. A wide range of mutations in the prothrombin gene contributes to its deficiency resulting in coagulation disorders like dysprothrombinemia and hypoprothrombinemia. High levels of thrombin elicit neurotoxicity in dopaminergic neurons and contributes to the progression of Parkinson′s disease. Altered thrombin levels modulates the coagulation pathway in multiple sclerosis. Patients with coronary artery disease (CAD) show elevated levels of thrombin. Thrombin accumulation in neurofibrillary tangles of the brain may contribute to the aggregation of tau protein and pathophysiology of Alzheimer disease.

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Analysis Note

The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Bezpečnostní informace

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Skladovací třída: 13 - Non Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable