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T1895

Thymidine

powder, BioReagent, suitable for cell culture

Synonyma:

1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil, 1-(2-Deoxy-β-D-ribofuranosyl)thymine, 2′-Deoxythymidine, Thymine deoxyriboside, dT

Přihlásit pro zobrazení organizačních a smluvních cen.

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Vám/Skladová položkaDostupnostCena
1 g
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
2 520,00 Kč
5 g
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4 330,00 Kč
10 g
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
7 860,00 Kč
25 g
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
16 900,00 Kč

O této položce

Empirický vzorec (Hillův zápis):
C10H14N2O5
Číslo CAS:
50-89-5
Molekulová hmotnost:
242.23
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
24899996
EC Number:
200-070-4
Beilstein/REAXYS Number:
89285
MDL number:
MFCD00006537
Assay:
≥99%
Biological source:
synthetic (organic)
Form:
powder
Solubility:
water: 50 mg/mL, clear, colorless

2 520,00 Kč

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biological source

synthetic (organic)

Quality Segment

300

product line

BioReagent

assay

≥99%

form

powder

technique(s)

cell culture | mammalian: suitable

mp

186-188 °C (lit.)

solubility

water: 50 mg/mL, clear, colorless

SMILES string

CC1=CN([C@H]2C[C@H](O)[C@@H](CO)O2)C(=O)NC1=O

InChI

1S/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7+,8+/m0/s1

InChI key

IQFYYKKMVGJFEH-XLPZGREQSA-N

Gene Information

human ... DTYMK(1841)

General description

Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine.

Application

Thymidine can be used in HT and HAT cocktails for hybridoma fusion, selection and cloning. It has been used:
  • as a nucleotide source in minimal essential medium to evaluate the outgrowth of the iSCNT-derived blastocysts
  • as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) selection medium used for the production of anti-neurogranin (NRGN) antibodies
  • to prepare THMG (thymidine, hypoxanthine, glycine, methotrexate) and THG stock solution for cleansing mouse lymphoma cells

Biochem/physiol Actions

Thymidine is useful in cell synchronization during S-phase. In the salvage pathway of pyrimidines, pyrimidine phosphorylase reversibly converts thymine to thymidine.

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Tato položka
T9250606089270
Thymidine powder, BioReagent, suitable for cell culture

Sigma-Aldrich

T1895

Thymidine

Thymidine ≥99%

Sigma-Aldrich

T9250

Thymidine

Thymidine

Millipore

6060

Thymidine

Thymidine ≥99.0% (HPLC)

Sigma-Aldrich

89270

Thymidine

biological source

synthetic (organic)

biological source

synthetic (organic)

biological source

-

biological source

synthetic

Quality Level

300

Quality Level

300

Quality Level

100

Quality Level

200

form

powder

form

powder

form

solid

form

powder

assay

≥99%

assay

≥99%

assay

≥98% (HPLC)

assay

≥99.0% (HPLC)

solubility

water: 50 mg/mL, clear, colorless

solubility

water: 50 mg/mL, clear, colorless

solubility

water: 10 mg/mL

solubility

hot water: 1 g/10 mL

mp

186-188 °C (lit.)

mp

186-188 °C (lit.)

mp

-

mp

185-190 °C, 186-188 °C (lit.)

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Skladovací třída

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–5 of 5 Questions  

  1. How can I sterilize a solution of Product T1895, Thymidine?

    1 answer

    1. Solutions can be sterile filtered using 0.2 μm filters (low protein binding). Do not autoclave solutions.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. How can I solubilize Product T1895, Thymidine?

    1 answer

    1. This product is soluble in water (50 mg/mL), yielding a clear, colorless solution with heating. For aqueous concentrations higher than 50 mg/mL, solubility can be enhanced with the addition of a small amount of NaOH solution. Add the base drop wise with slow stirring. Thymidine is also soluble in methanol, hot alcohol, hot acetone, hot ethyl acetate, pyridine, glacial acetic acid, and is sparingly soluble in hot chloroform.

      Helpful?

  4. How long can I store media supplemented with Product T1895, Thymidine?

    1 answer

    1. Thymidine is a component of Nutrient Mixture F-12 at 0.73mg/L.  This media has an expiration date of 18 months from date of formulation.  You will need to determine if this is appropriate for your application.

      Helpful?

  5. What concentration of Product T1895, Thymidine, is used for testing this product for cell culture applications?

    1 answer

    1. The tested working concentration for cell culture for Thymidine is 8 mg/L.

      Helpful?

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