SRP5009

CDK1/CyclinB1, active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonyma: CCNB, CCNB1, CDC2
• NACRES: NA.32
• UNSPSC Code: 12352200
• Specific activity: 12-16 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells

Vlastnosti

• recombinant: expressed in baculovirus infected Sf9 cells
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 12-16 nmol/min·mg
• mol wt: ~58 kDa (CDK1), ~75 kDa (CyclinB1)
• NCBI accession no.: NM_001786, NM_031966
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CCNB1(891), CDK1(983)

Popis

General description

The cyclin B1 (CCNB1) gene is mapped to human chromosome 5q13.2. The gene codes for a 62kDa protein containing a classical cyclin box domain. CCNB1 shuttles between the nucleus and cytoplasm until prophase begins. It is a member of the cyclin family and is expressed at low levels in normal cells. CDK1 (cyclin-dependent kinase 1) belongs to the Ser/Thr protein kinase family. The gene encoding it is localized on human chromosome 10q21.

Biochem/physiol Actions

The cyclin B1 (CCNB1) and cyclin-dependent kinase 1 (CDK1) complex plays a crucial role in the regulation of mitosis. Elevated expression of CCNB1 has been observed in various types of human cancers including breast, lung, colon, prostate and head and neck cancers. Thus, this protein can be considered as a potent target for cancer vaccines.In addition, CCNB1 also serves as a prognostic biomarker for estrogen-receptor-positive (ER+) breast cancer. CDK1 (cyclin-dependent kinase 1) controls various mitotic processes like reorganization of the cytoskeleton and segregation of chromosomes. It is essential for the multiplication of mammalian cells.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• Skladovací třída: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable