SRP0122

LSD1 Active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Synonyma: Amine oxidase (flavin containing) domain 2 (AOF2), BRAF35-HDAC complex protein BHC110, KIAA0601, Lysine (K)-specific demethylase 1 (LSD1), Lysine-specific histone demethylase 1A (KDM1A)
• NACRES: NA.32
• UNSPSC Code: 12352202

Vlastnosti

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥70% (SDS-PAGE)
• form: aqueous solution
• mol wt: 103 kDa
• packaging: pkg of 50 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_015013
• UniProt accession no.: O60341
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... KDM1A(23028)

Popis

General description

Human LSD1, AOF2, amine oxidase, flavin containing, domain 2, GenBank Accession No. NM_015013, amino acids 158-end with N-terminal ST tag, MW = 103 kDa, expressed in E. coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Other Notes

One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.

Bezpečnostní informace

• Skladovací třída: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable