SRE0018

Lyticase from Arthrobacter Luteus

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥2000 units/mg protein

• Synonyma: Lyticase from Arthrobacter luteus
• Číslo CAS: 37340-57-1
• UNSPSC Code: 12352204
• NACRES: NA.32
• MDL number: MFCD00131560
• Specific activity: ≥2000 units/mg protein
• Biological source: bacterial (Arthrobacter luteus)
• Concentration: 20 % protein Biuret

Vlastnosti

• biological source: bacterial (Arthrobacter luteus)
• Quality Segment: 400
• form: lyophilized powder
• specific activity: ≥2000 units/mg protein
• composition: Protein, ≥20% biuret
• concentration: 20 % protein Biuret
• color: light brown
• input: plant, fungi and insect sample(s)
• application(s): diagnostic assay manufacturing
• shipped in: wet ice
• storage temp.: −20°C

Popis

General description

Lyticase is an enzyme obtained from Arthrobacter Luteus that can extract DNA from yeast by inducing partial spheroplast formation which are later lysed to release DNA. It is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Lyticase contains β-(1→3)-glucan laminaripentaohydrolase with additional β-(1→3)-glucanase, protease, and mannanase activities.

Application

For isolation of nucleic acids, lyticase is used in the lysis of yeast cell walls (e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species).

Biochem/physiol Actions

Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Features and Benefits

Features:

1. Qualified Raw Materials: Products that meet the stringent requirements of the Elevated Assurance Program, ensuring compliance with regulatory standards.
2. Comprehensive Documentation: Includes essential documents such as certificates, impurity profiles, and declarations to support audits and risk assessments.
3. M-Clarity^™ Quality Attributes: Products are classified within the MQ300 quality segment or higher, demonstrating superior quality assurance. M-Clarity^™ Program
4. Confirmed Process Controls: Established processes that ensure consistency and reliability in manufacturing.
5. Enhanced Change Notification: Timely updates on any changes to product specifications or processes, ensuring transparency and compliance.
6. Freely Available Dossiers: Access to robust, ready-to-present product dossiers that include:
   1. Site quality assessment
   2. ISO 9001 certificate
   3. Detailed product specifications
   4. Relevant declarations/statements (e.g., animal origin content, BSE/TSE, nitrosamine)

Benefits:

1. Regulatory Compliance: Helps you meet compliance standards effectively, reducing the risk of regulatory issues.
2. Audit Readiness: Instills confidence during audits with comprehensive documentation and confirmed process controls, demonstrating your commitment to high-quality products.
3. Operational Transparency: Supports supply chain transparency, allowing you to track and verify the quality of raw materials.
4. Enhanced Support: Provides exceptional quality-related information and tailored assistance to meet your specific needs.
5. Risk Mitigation: Facilitates thorough risk assessments with detailed documentation, helping you manage potential compliance challenges.
6. Informed Decision-Making: Access to clear and comprehensive product information enables better decision-making and enhances operational efficiency.

Other Notes

One unit will produce a ΔA₈₀₀ of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Legal Information

M-CLARITY is a trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• Piktogramy: GHS08
• Výstražné slovo: Danger
• Kódy nebezpečí: H334
• Bezpečnostní pokyny: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Skladovací třída: 11 - Combustible Solids
• Co se děje?: WGK 3
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable