SML1661

Bafilomycin A1

from Streptomyces griseus, ≥90% (HPLC), DMSO solution, V-ATPase inhibitor

• Synonyma: BafA1
• Empirický vzorec (Hillův zápis): C₃₅H₅₈O₉
• Číslo CAS: 88899-55-2
• Molekulová hmotnost: 622.83
• UNSPSC Code: 12352200
• NACRES: NA.77
• Form: DMSO solution

Vlastnosti

• Název produktu: Bafilomycin A1 Ready Made Solution, 0.16 mM in DMSO, from Streptomyces griseus
• biological source: Streptomyces griseus
• Quality Segment: 200
• form: DMSO solution
• concentration: 0.16 mM in DMSO
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: O1[C@@H]([C@H]([C@@H](C[C@]1(O)[C@H]([C@H](O)[C@@H]([C@H]2OC(=O)\C(=C\C(=C\[C@H]([C@H]([C@H](C\C(=C\C=C\[C@@H]2OC)\C)C)O)C)\C)\OC)C)C)O)C)C(C)C
• InChI: 1S/C35H58O9/c1-19(2)32-24(7)27(36)18-35(40,44-32)26(9)31(38)25(8)33-28(41-10)14-12-13-20(3)15-22(5)30(37)23(6)16-21(4)17-29(42-11)34(39)43-33/h12-14,16-17,19,22-28,30-33,36-38,40H,15,18H2,1-11H3/b14-12+,20-13+,21-16+,29-17-/t22-,23+,24-,25-,26-,27+,28-,30-,31+,32+,33+,35+/m0/s1
• InChI key: XDHNQDDQEHDUTM-JQWOJBOSSA-N

Popis

General description

Bafilomycin A1, a macrolide antibiotic, belongs to the pleomacrolides family. It acts as a potent and selective inhibitor of vacuolar-type H⁺-ATPase. BafA1 can inhibit the viability of MG63 osteosarcoma cells. It can also stimulate mitochondrial dysfunction. BafA1 inhibits the proliferation of different types of cancer cells.

Application

Bafilomycin A1 has been used:

• as an endosome acidification inhibitor to study the importance of endosome acidification in the extracellular vesicle uptake and cytosolic release of stably expressing NanoLuc luciferase-tagged Hsp70 (NLuc-Hsp70) in HeLa cells[1]
• as a vacuolar-type H⁺-ATPase (V-ATPase) inhibitor to study its effects on autophagic turnover of light chain 3 β (LC3-II) in mice[2]
• as an autophagy inhibitor to study its effects on primary rat liver sinusoidal endothelial cells (LSECs) defenestration[3]

Biochem/physiol Actions

Bafilomycin A1 inhibits autophagy. It may exhibit anti-tumorigenic, anti-parasitic, and anti-neurodegenerative effects.[4] Bafilomycin A1, found in lysosomes and endosomes prevents the acidification of these cell organelles.[5] It also participates in blocking autophagosome-lysosome fusion and autolysosome acidification, steps necessary for maintaining the autophagic flux and cellular homeostasis.[6]

Bafilomycin A1 is a macrolide antibiotic.

Bafilomycin A1 is a macrolide antibiotic. Bafilomycin A1 acts as a potent and selective inhibitor of vacuolar-type H⁺-ATPase.

Other Notes

Bafilomycin A1 solution is provided in concentration of 0.16 mM. Typical concentrations for use in cell culture are 50-100 nM.

Bezpečnostní informace

• Skladovací třída: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: 188.6 °F - closed cup
• flash_point_c: 87 °C - closed cup