SAE0104

BmrA (Bacilus Subtilis Multidrug Resistance Abc Transporter Atp-Binding Protein)

• UNSPSC Code: 12352202
• NACRES: NA.83

Vlastnosti

• recombinant: expressed in E. coli
• description: C-Terminal six-histidine tagged
• assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• mol wt: 64.5 kDa
• concentration: 0.3 mg/mL
• UniProt accession no.: O06967
• shipped in: dry ice
• storage temp.: −70°C

Popis

Biochem/physiol Actions

BmrA is an efflux protein and ABC transporter that acts as an ATP-dependent drug efflux pump.

Preparation Note

Formulated in 50mM Tris-Cl pH 8.0, 100mM NaCl, 0.01% DDM, 10% glycerol.

Storage and Stability

Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot into single use aliquots. Store remaining undiluted protein in aliquots at -80°C. Note: avoid freeze/thaw cycles.

Substrates

MSSSHHHHHHMPTKKQKSKSKLKPFFALVRRTNPSYGKLAFALALSVVTTLVSLLIPLLTKQLVDGFSMSNLSGTQIGLIALVFFVQAGLSAYATYALNYNGQKIISGLRELLWKKLIKLPVSYFDTNASGETVSRVTNDTMVVKELITTHISGFITGIISVIGSLTILFIMNWKLTLLVLVVVPLAALILVPIGRKMFSISRETQDETARFTGLLNQILPEIRLVKASNAEDVEYGRGKMGISSLFKLGVREAKVQSLVGPLISLVLMAALVAVIGYGGMQVSSGELTAGALVAFILYLFQIIMPMGQITTFFTQLQKSIGATERMIEILAEEEEDTVTGKQIENAHLPIQLDRVSFGYKPDQLILKEVSAVIEAGKVTAIVGPSGGGKTTLFKLLERFYSPTAGTIRLGDEPVDTYSLESWREHIGYVSQESPLMSGTIRENICYGLERDVTDAEIEKAAEMAYALNFIKELPNQFDTEVGERGIMLSGGQRQRIAIARALLRNPSILMLDEATSSLDSQSEKSVQQALEVLMEGRTTIVIAHRLSTVVDADQLLFVEKGEITGRGTHHELMASHGLYRDFAEQQLKMNADLENKAG

Bezpečnostní informace

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable