SAB5600096

Anti-His-Tag Chimeric antibody, Human Monoclonal

recombinant, expressed in HEK 293 cells, clone RMH01, purified immunoglobulin

• Synonyma: Anti-His tag antibody
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: RMH01, monoclonal, recombinant monoclonal
• Technique(s): capture ELISA: 0.1-2 μg/mL, western blot: 0.1-1 μg/mL
• Application: ELISA (c), WB
• Citations: 4

Vlastnosti

• recombinant: expressed in HEK 293 cells
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: RMH01, monoclonal, recombinant monoclonal
• form: buffered aqueous glycerol solution
• technique(s): capture ELISA: 0.1-2 μg/mL, western blot: 0.1-1 μg/mL
• isotype: IgG
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Popis

General description

Hexahistidine tag (His-tag) consists of four to six histidines fused genetically. It is widely used to purify proteins by immobilized metal-ion affinity chromatography (IMAC).

Immunogen

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Biochem/physiol Actions

This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Skladovací třída: 10 - Combustible liquids
• Co se děje?: WGK 2