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S4195

Sigma-Aldrich

β-Secretase human

recombinant, expressed in HEK 293 cells (C-terminal FLAG tagged), extracellular domain, ≥10,000 units/mg protein, ≥90% (SDS-PAGE), buffered aqueous solution

Synonyma:

Asp-2, BACE1, Memapsin-2

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
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About This Item

MDL number:
MFCD03702801
UNSPSC Code:
12352204
NACRES:
NA.32

recombinant

expressed in HEK 293 cells (C-terminal FLAG tagged)

Quality Level

200

assay

≥90% (SDS-PAGE)

form

buffered aqueous solution

specific activity

≥10,000 units/mg protein

UniProt accession no.

P56817

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... BACE1(23621)

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Application

Human β-secretase or BACE1 has been used to study the binding reaction between amyloid precursor protein and β-secretase. It has also been used in BACE1 assay.

Biochem/physiol Actions

BACE1 (β−Secretase or β−Site APP-Cleaving Enzyme) is a transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide (Aβ). The accumulation of Aβ in the brain is a primary cause for the progression of Alzheimer′s, therefore BACE1 is a target for inhibitor drug discovery.
BACE1 (β-site APP-cleaving enzyme 1) is a transmembrane aspartyl protease responsible for cleaving the amyloid precursor protein (APP). BACE1-mediated cleavage of amyloid precursor protein (APP) is the first step during the generation of pathogenic amyloid-β peptides, hence making it the major drug target for Alzheimer′s disease (AD). Increased levels of enzymatic activity of BACE1 was found in cerebrospinal fluid from patients having both mild cognitive impairment (MCI) and AD, thereby suggesting the importance of BACE1 enzymatic activity in conversion of MCI to AD.

Unit Definition

One unit will hydrolyze 1.0 picomole of 7-methoxycoumarin-4-acetyl-[Asn670, Leu671]-Amyloid β/A4 Precursor Protein 770 Fragment 667-676-(2,4-dinitrophenyl)-Lys-Arg-Arg amide substrate per 1 minute at pH 4.5 at 37 °C.

Physical form

Solution in 20 mM Hepes, pH 7.4, 125 mM NaCl.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Osvědčení o analýze (COA)

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DAPT ≥98% (HPLC), solid

Sigma-Aldrich

D5942

DAPT

Giovanni Zuliani et al.
Journal of neurochemistry, 159(3), 629-637 (2021-09-18)
Beta-secretase 1 (BACE1) is considered as the key enzyme in amyloid-β formation. Previous works suggest that high BACE1 activity may be present in brain, cerebrospinal fluid and serum of patients with late-onset Alzheimer's disease (LOAD) as well as mild cognitive
Dennis J Selkoe
Nature cell biology, 6(11), 1054-1061 (2004-11-02)
The salutary intersection of fundamental cell biology with the study of disease is well illustrated by the emerging elucidation of neurodegenerative disorders. Novel mechanisms in cell biology have been uncovered through disease-orientated research; for example, the discovery of presenilin as
cceleration of deposition of peptide on ultrasonically formed nucleus studied by wireless quartz-crystal-microbalance biosenso
Hirotsugu Ogi
Biosensors, 40(1) (2013)
Hirotsugu Ogi et al.
Biosensors & bioelectronics, 40(1), 200-205 (2012-08-04)
High-frequency (~ 55 MHz) wireless quartz-crystal microbalance biosensor was used for studying heterogeneous deposition behavior of Aβ(1-40) peptide on Aβ(1-42) nuclei, which were grown under the stirring agitation and 200-kHz ultrasonication at pH 2.2, 4.6, and 7.4. The deposition reaction
Acceleration of deposition of peptide on ultrasonically formed nucleus studied by wireless quartz-crystal-microbalance biosensor
Hirotsugu Ogi
Biosensors And Bioelectronics (2013)
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