R7017

Resazurin sodium salt

powder, BioReagent

• Synonyma: 7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt
• Empirický vzorec (Hillův zápis): C₁₂H₆NNaO₄
• Číslo CAS: 62758-13-8
• Molekulová hmotnost: 251.17
• UNSPSC Code: 12352207
• NACRES: NA.75
• PubChem Substance ID: 24899387
• EC Number: 263-718-5
• Beilstein/REAXYS Number: 3794971
• MDL number: MFCD00005036

Vlastnosti

• product line: BioReagent
• Quality Segment: 200
• form: powder
• composition: Dye content, ~80%
• pH: 3.8-6.5, orange to violet
• solubility: H₂O: soluble
• λmax: 600 nm
• SMILES string: [Na+].[O-]c1ccc2c(OC3=CC(=O)C=CC3=[N+]2[O-])c1
• InChI: 1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1
• InChI key: IVGPGQSSDLDOLH-UHFFFAOYSA-M

Popis

Application

For the measurement of metabolic activity and proliferation of living cells. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).

Resazurin sodium salt has been used in viability assay:

• to measure cell viability of A. fumigatus
• to assess cell proliferation
• to determine A. fumigatus viability in high-throughput screenings of microbial natural extracts

Biochem/physiol Actions

Resazurin is a blue, non-fluorescent dye originally used to test milk/biological fluids for bacterial and yeast contamination. It is also useful in sperm viability and semen quality test. Resazurin is applicable in cytotoxicity determination.

Bezpečnostní informace

• ppe: dust mask type N95 (US), Eyeshields, Gloves
• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable