R1153

RNase B Glycoprotein Standard from bovine pancreas

≥90% (SDS-PAGE), lyophilized powder

• Synonyma: Ribonuclease B from bovine pancreas, RNase B
• Číslo CAS: 9001-99-4
• UNSPSC Code: 12352204
• NACRES: NA.32
• EC Number: 232-646-6
• MDL number: MFCD00132184

Vlastnosti

• Název produktu: RNase B Glycoprotein Standard from bovine pancreas, Proteomics Grade
• grade: Proteomics Grade
• Quality Segment: 200
• assay: ≥90% (SDS-PAGE)
• form: lyophilized powder
• storage temp.: −20°C
• SMILES string: [nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
• InChI: 1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
• InChI key: CQOVPNPJLQNMDC-UHFFFAOYSA-N

Popis

Application

RNase B is a preferred substrate with PNGase F for demonstration of N-linked deglycosylation using SDS-PAGE or MALDI-MS. The activity of PNGase F is routinely assayed using RNase B by monitoring the pronounced mobility shift in 12% gels after deglycosylation. Proteomics Grade RNase B has been used as a source of N-glycans following enzymatic digestions and subsequent purification. It has also been used as a glycoprotein standard.

Analysis Note

RNAse B Glycoprotein Standard has been highly purified to remove contaminating RNase A.

Other Notes

Bovine pancreatic RNase B is a glycoprotein that contains only N-linked glycans. It is a globular protein composed of a single domain that occurs naturally as a lesser component in mixture along with ribonuclease A (RNase A), which is the non-glycosylated core form. RNase B contains a single glycosylation site at Asn³⁴ at which from five to nine mannose residues are attached to the chitobiose core, i.e. Man_5-9GlcNAc₂. Due to the heterogeneity in the glycosylation at Asn³⁴, RNase B exists as five glycosylated varients, with a molecular weight of approx. 15 kDa.

Bezpečnostní informace

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)