This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
P7032
Pyruvate Dehydrogenase from porcine heart
buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry)
Synonym(s):
PDH, Pig heart pyruvate dehydrogenase
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
10 units | Check Cart for Availability | $412.00 |
25 units | Check Cart for Availability | $743.00 |
About This Item
Specific activity:
0.1-1.0 units/mg protein (Lowry)
Concentration:
≥5.0 mg/mL
$412.00
Check Cart for Availability
form
buffered aqueous glycerol solution
Quality Segment
specific activity
0.1-1.0 units/mg protein (Lowry)
concentration
≥5.0 mg/mL
impurities
polyethylene glycol, trace
foreign activity
α-ketoglutarate dehydrogenase ≤15%
shipped in
dry ice
storage temp.
−20°C
Application
Pyruvate dehydrogenase contributes to linking the glycolysis metabolic pathway to the citric acid cycle and releases energy via NADH. It is used to study how aerobic training effects pyruvate dehydrogenase [1]. It is also used to study the interaction between pyruvate dehydrogenase kinase and the pyruvate dehydrogenase complex[2] as well as the function and regulation of the pyruvate dehydrogenase complex. Product P7032 is provided in a buffered aqueous glycerol solution and is from porcine heart.
The enzyme has been used in a primary enzyme assay to test the ability of certain compounds to reduce PDK- (pyruvate dehydrogenase kinase) catalyzed PDC (pyruvate dehydrogenase complex) inactivation.[3]
Biochem/physiol Actions
Pyruvate dehydrogenase is involved in the decarboxylation of pyruvate and reductive acetylation of lipoic acid within the pyruvate dehydrogenase complex (PDC). Pyruvate dehydrogenase kinase (PDK) inactivates pyruvate dehydrogenase while pyruvate dehydrogenase phosphatase activates pyruvate dehydrogenase[4].
Physical form
Supplied as a 50% glycerol solution containing ~9 mg/mL bovine serum albumin, 30% sucrose, 1.5 mM EDTA, 1.5 mM EGTA, 1.5 mM 2-mercaptoethanol, 0.3 TRITON® X-100, 0.003% sodium azide, and 15 mM potassium phosphate, pH 6.8
Other Notes
One unit will convert 1.0 μmole of β-NAD to β-NADH per min at pH 7.4 at 30 °C in the presence of saturating levels of Coenzyme A.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity 0.1-1.0 units/mg protein (Lowry) | specific activity 0.1-1.0 units/mg protein (Lowry) | specific activity 600-1000 units/mg protein (biuret) | specific activity ≥300 units/mg protein |
| form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form lyophilized |
| concentration ≥5.0 mg/mL | concentration - | concentration - | concentration - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in ambient |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
Hi I want to know what is the expiration for this product? e.g. 1 year, 2 years,3 years. Thanks, Aviv.
1 answer-
Helpful?
-