P6246

Anti-PABP antibody, Mouse monoclonal

clone 10E10, purified from hybridoma cell culture

• Synonym(s): Anti-Poly(A)-Binding Protein
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD06412253
• Conjugate: unconjugated
• Clone: 10E10, monoclonal
• Application: ARR, ICC, IP, WB
• Citations: 19

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 10E10, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~69 kDa
• species reactivity: hamster, canine, bovine, Xenopus, human, monkey
• technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, microarray: suitable, western blot: 0.5-1 μg/mL using whole cell extracts of HEK 293T cells
• isotype: IgG1
• UniProt accession no.: P11940
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PABPC1(26986)

Description

General description

Monoclonal Anti-PABP (mouse IgG1 isotype) is derived from the 10E10 hybridoma produced by the fusion of mouse myeloma cells (SP2/0) and splenocytes from BALB/c mice immunized with recombinant human PABP. Poly(A) binding protein (PABP) is highly conserved between different organisms especially at the amino-terminal region of the protein that contains four RNA-binding domains (RBDs).

Poly(A) binding protein (PABP) possesses RNA recognition motifs (RRMs), a proline-rich region and a PABP C-terminal domain. The gene encoding this protein is localized on human chromosome 8q22.3.

Immunogen

recombinant human PABP.

Application

Monoclonal Anti-PABP antibody produced in mouse has been used in:

• Immunoblotting
• Immunostaining[1]
• Immunoprecipitation
• Enzyme linked immunosorbent assay(ELISA)
• Immunocytochemistry

Monoclonal Anti-PABP antibody produced in mouse has been used for immunofluorescence.

Biochem/physiol Actions

Poly(A) binding protein (PABP) has a role in mRNA translation and stability. It modulates gene expression. The protein binds to the poly(A) tail, bridges the ends of mRNA and forms a closed loop. This might help in translation initiation by promoting ribosome recruitment.

Poly(A) binding protein (PABP) is found in excess, relative to the amount of cytoplasmic poly(A) (three fold) and binds to its poly(A) target at high affinity (Kd of 7nM). The eukaryotic initiation factor, EIF4F complex, interacts with PABP indirectly via the PAIP-1 protein (PABP-interacting protein-1). As a consequence, an interaction between the 5′ and 3′ ends of mRNA occurs. This proximity between the mRNA ends contributes to its stability and enhances translation, since terminating ribosomes may start translation again.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable