N5386

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

• Synonyma: Endonuclease micrococcal, MNase, Micrococcal endonuclease
• Číslo CAS: 9013-53-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-748-0
• MDL number: MFCD00131731
• Číslo EC: 3.1.31.1 (BRENDA, IUBMB)
• Specific activity: 100-300 units/mg protein
• Biological source: Staphylococcus aureus

Vlastnosti

• biological source: Staphylococcus aureus
• Quality Segment: 200
• form: powder
• specific activity: 100-300 units/mg protein
• composition: Protein, ≥40% E1%/280 (, Balance primarily sodium acetate)
• technique(s): DNA extraction: suitable, DNA purification: suitable
• suitability: suitable for molecular biology
• application(s): cell analysis
• storage temp.: −20°C
• Gene Information: Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

Popis

Application

Nuclease micrococcal from Staphylococcus aureus has been used in a study to monitor hybridization reactions with DNA. It has also been used in a study to investigate the optimal conditions for assay of staphylococcal nuclease.

Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes.

Biochem/physiol Actions

Hydrolyzes 5′-phosphodiester bonds of DNA.

Other Notes

Note: One μmolar unit = approx. 85 A₂₆₀ units, where an A₂₆₀ unit is equivalent to a ΔA₂₆₀ of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)